##Background My digital pictures can be read into a computer program like Matlab or R as a m x n x 3 matrix where m x n is the number of pixels observed by each of the three (red, green, and blue) sensors, and each cell in the matrix has a number from 1-255 that reflects the brightness observed by the sensor. I would like to use this information to obtain an objective measure of greenness in a photograph, because I want to attempt to correlate greenness to plant growth (imagine one picture per day of a corn field). [Previous work][1] in this direction has had some success by calculating an index of green either as - green % = green/(blue + red) or - green divergence = 2*green - red - blue from webcam images for each of the m x n pixels, but there was no control over the aperture or incident radiation (solar angle). note that I am not looking for an 'absolute' measure of greenness, the scale and distribution of the number does not matter - it just has to provide a consistent relative measure of greenness. ##Question Can I use my SLR to get a robust measure of greenness that is invariant with any or all of the following: - cloud cover? - time of day? - day of year? (this is the only requirement) - proportion of sky / ground in the background? ##Current Status I have come up with the following ideas, but I am not sure which would be necessary, or which ones would have no effect on the ratio of green/(red + blue) 1. take a picture of a white piece plastic, and use this image to normalize the other values 2. Fix aperture 4. Fix shutter speed 2. set the white balance using a white piece of paper 3. Take all photos from the same angle 4. Take all photos at solar noon [1]: http://www.forest.sr.unh.edu/richardson/RichardsonOecologia2007.pdf