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What is the purpose of pre-focusing on a Canon AF35m ii?

I am HIGHLY confused as to what this means. The camera says to press the shutter once to prefocus & another time to take the actual shot . Why do i have to move after ive pressed the shutter ...
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Why is there a difference between the shutter lag times for prefocus and for manual focus?

In my lab I have two cameras, the Canon T2i and the Canon T3i, which are without their standard lenses. Instead they are mounted on a microscope. To capture the images of my samples, I set the camera ...