While retouching I saved my file as a tif and then flatten it and saved as a jpeg. I accidentally saved the tif again while it was flattened with no layers. Is there a way to recover my tif file with the layers?
If you are still in the program, you might be able to undo.
If you have some kind of automatic backups or versioning filesystem installed, you might be able to recover from that.
Otherwise, sorry, no.