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Optics is the science of light, and in photography often refers more specifically to to the properties and construction of lenses or the technical aspects of light sensors.

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How to calculate the magnification factor of an object to its image?

I have an object of size 37.5um x 37.5um, and captured a 640 x 480 resolution image of the object with a CMOS sensor. I see that the object is around 200 x 200 pixels in the image. How do I calculate ...